High Magnetic Field Laboratory of the Chinese Academic of Science
Anhui University Institutes of Physical Science and Information Technology
From High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences,
Hefei Institutes of Physical Science Chinese Academy of Sciences
Hefei Institutes of Physical Science Chinese Academy of Sciences High Magnetic Field Laboratory
This study was supported by National Key R&D Program of China (2023YFB3507004), National Natural Science Foundation of China (U21A20148), International Partnership Program of Chinese Academy of Sciences (116134KYSB20210052), Anhui Provincial Natural Science Foundation (2308085QE183, 2308085QE181), CASHIPS Director’s Fund (YZJJ2024QN44, YZJJ2023QN43), Heye Health Technology Chong Ming Project (HYCMP2021010), China Post-doctoral Science Foundation (2023M743536) and Science Research Fund for Post-doctoral in Anhui Province (2023B669).
Acetaminophen (APAP) is the most commonly used mild analgesic and antipyretic drug worldwide. Its overdoses account for 46% of all acute liver failures in the USA and 40–70% in Europe. However, the only approved pharmacological treatment is the antioxidant N-acetylcysteine (NAC), but it does not work well with advanced liver injury or administrates at later stage. Here, we found that a moderate intensity static magnetic field (SMF) treatment can reduce the mice death rate of high-dose APAP from 40% to 0%, and it works at both earlier liver injury stage and the later liver recovery stage. At the early liver injury stage, SMF can effectively decrease APAP-induced oxidative stress, reduce free radical levels and liver damage. Multiple oxidative stress markers were all reduced, while the antioxidant glutathione (GSH) level was increased by SMF. At the later liver recovery stage, the vertically downward SMF can increase the DNA synthesis and hepatocyte proliferation. Moreover, the combination of NAC and SMF can significantly reduce the high-dose APAP-induced liver damage and increase liver recovery, even at 24 hours after APAP overdose, when NAC alone does not work well anymore. Therefore, our study provides a non-invasive nonpharmaceutical physical tool that has dual roles in the injury and repair stages after APAP overdose. It can work as an alternative or combinational strategy with NAC to prevent or minimize liver damage induced by APAP, and maybe other toxin overdose as well.