Turn off MathJax
Article Contents
Qiang Sun, Xingchen Liu, Chunyang Li, Yuzhuo Li, Yan Wang, Yanhong Nie, Yuting Xu, Xiaotong Zhang, Yong Lu. Generation of mitochondria replacement monkeys by female pronuclear transfer. Zoological Research. doi: 10.24272/j.issn.2095-8137.2023.287
Citation: Qiang Sun, Xingchen Liu, Chunyang Li, Yuzhuo Li, Yan Wang, Yanhong Nie, Yuting Xu, Xiaotong Zhang, Yong Lu. Generation of mitochondria replacement monkeys by female pronuclear transfer. Zoological Research. doi: 10.24272/j.issn.2095-8137.2023.287

Generation of mitochondria replacement monkeys by female pronuclear transfer

doi: 10.24272/j.issn.2095-8137.2023.287
  • Received Date: 2023-09-05
  • Accepted Date: 2023-12-14
  • Rev Recd Date: 2023-11-22
  • Published Online: 2023-12-26
  • Mutations in mitochondrial DNA (mtDNA) are maternally inherited and may cause severe disorders. Mitochondrial replacement therapy has been a promising option to prevent the transmission of mtDNA diseases, which include spindle transfer, polar body transfer and pronuclear transfer. The pronuclear transfer has been used for generating mitochondrial replaced mouse individuals and human embryos. In contrast, none of the primate individuals have been generated through pronuclear transfer. In this study, we successfully generated four healthy cynomolgus monkeys (Macaca fascicularis) using female pronuclear transfer, all of which have survived for more than two years and showed small amounts of mtDNA carryover (3.8%-6.7%), as well as relatively stable mtDNA heteroplasmy dynamics during development. Our non-human primate model demonstrates that pronuclear transfer has great potential to reduce the risk of inherited mtDNA disease, which provides a useful preclinical research model for human mitochondrial replacement therapy.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (123) PDF downloads(7) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return