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Xin-Chun Yang, Xiao-Long Wu, Wen-Hao Li, Xiao-Jie Wu, Qiao-Yan Shen, Yun-Xiang Li, Sha Peng, Jin-Lian Hua. OCT6 inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation. Zoological Research, 2022, 43(6): 911-922. doi: 10.24272/j.issn.2095-8137.2022.220
Citation: Xin-Chun Yang, Xiao-Long Wu, Wen-Hao Li, Xiao-Jie Wu, Qiao-Yan Shen, Yun-Xiang Li, Sha Peng, Jin-Lian Hua. OCT6 inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation. Zoological Research, 2022, 43(6): 911-922. doi: 10.24272/j.issn.2095-8137.2022.220

OCT6 inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation

doi: 10.24272/j.issn.2095-8137.2022.220
#Authors contributed equally to this work
Funds:  This work was supported by the National Natural Science Foundation of China (32072806), Shaanxi Province Science and Technology Innovation Team Project (2019TD-036), and Shaanxi Province Science and Technology Project (2022NY-044)
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  • Corresponding author: E-mail: jinlianhua@nwsuaf.edu.cn
  • Received Date: 2022-07-01
  • Accepted Date: 2022-09-01
  • Published Online: 2022-09-02
  • As a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 (OCT6) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of OCT6 in piPSCs. We found that piPSCs overexpressing OCT6 maintained colony morphology and pluripotency under differentiation conditions, with a similar gene expression pattern to that of non-differentiated piPSCs. Functional analysis revealed that OCT6 attenuated the adverse effects of extracellular signal-regulated kinase (ERK) signaling pathway inhibition on piPSC pluripotency by activating phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) signaling activity. Our research sheds new light on the mechanism by which OCT6 promotes PSC maintenance.
  • #Authors contributed equally to this work
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