Chun-Xue Niu, Jia-Wei Li, Xiao-Li Li, Lin-Lin Zhang, Yan Lang, Zhen-Bo Song, Chun-Lei Yu, Xiao-Guang Yang, Hai-Feng Zhao, Jia-Ling Sun, Li-Hua Zheng, Xue Wang, Ying Sun, Xiao-Hong Han, Guan-Nan Wang, Yong-Li Bao. 2024. PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality. Zoological Research, 45(5): 1037-1047. DOI: 10.24272/j.issn.2095-8137.2023.388
Citation: Chun-Xue Niu, Jia-Wei Li, Xiao-Li Li, Lin-Lin Zhang, Yan Lang, Zhen-Bo Song, Chun-Lei Yu, Xiao-Guang Yang, Hai-Feng Zhao, Jia-Ling Sun, Li-Hua Zheng, Xue Wang, Ying Sun, Xiao-Hong Han, Guan-Nan Wang, Yong-Li Bao. 2024. PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality. Zoological Research, 45(5): 1037-1047. DOI: 10.24272/j.issn.2095-8137.2023.388

PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality

  • Serine protease 50 (PRSS50/TSP50) is highly expressed in spermatocytes. Our study investigated its role in testicular development and spermatogenesis. Initially, PRSS50 knockdown was observed to impair DNA synthesis in spermatocytes. To further explore this, we generated PRSS50 knockout (Prss50−/−) mice (Mus musculus), which exhibited abnormal spermatid nuclear compression and reduced male fertility. Furthermore, dysplastic seminiferous tubules and decreased sex hormones were observed in 4-week-oldPrss50−/− mice, accompanied by meiotic progression defects and increased apoptosis of spermatogenic cells. Mechanistic analysis indicated that PRSS50 deletion resulted in increased phosphorylation of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and elevated levels of MAP kinase phosphatase 3 (MKP3), a specific ERK antagonist, potentially accounting for testicular dysplasia in adolescentPrss50−/− mice. Taken together, these findings suggest that PRSS50 plays an important role in testicular development and spermatogenesis, with the MKP3/ERK signaling pathway playing a significant role in this process.
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