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项辉, 罗剑文, Sylvia ANTON, Bill S.HAN.SSON. 2001: Agrotis segetum雄蛾触角叶性信息素反应神经元对电刺激触角神经的反应. 动物学研究, 22(3): 181-186.
引用本文: 项辉, 罗剑文, Sylvia ANTON, Bill S.HAN.SSON. 2001: Agrotis segetum雄蛾触角叶性信息素反应神经元对电刺激触角神经的反应. 动物学研究, 22(3): 181-186.
XIANG Hui, LUO Jian-Wen, Sylvia ANTON, Bill S.HANSS. 2001. Electrophysiological Characteristics of Neurons Responding to Sex Pheromone in the Antennal Lobe of the Male Moth Agrotis segetum. Zoological Research, 22(3): 181-186.
Citation: XIANG Hui, LUO Jian-Wen, Sylvia ANTON, Bill S.HANSS. 2001. Electrophysiological Characteristics of Neurons Responding to Sex Pheromone in the Antennal Lobe of the Male Moth Agrotis segetum. Zoological Research, 22(3): 181-186.

Agrotis segetum雄蛾触角叶性信息素反应神经元对电刺激触角神经的反应

Electrophysiological Characteristics of Neurons Responding to Sex Pheromone in the Antennal Lobe of the Male Moth Agrotis segetum

  • 摘要: 为探讨电刺激Agrotis segetum雄蛾触角神经是否可以作为MGC中神经元的识别手段,采用细胞内电生理记录方法,共记录34个对性信息素有反应的MGC神经元,并测试了其中12个神经元对性信息素刺激的反应,22个神经元对性信息素刺激和电刺激的反应。结果表明,MGC神经元对性信息素及电刺激的反应模式基本一致,为一种双相反应模式。

     

    Abstract: The PN response pattern evoked by electrical shock would be interdependent with morphological results and could be used as descriptor of neurons in some moths. In this study,intracellular recordings were performed and 34 neurons in MGC of Agrotis segetum were recorded;all those neurons responded to sex pheromone.12 neurons just tested by sex pheromone,and 22 neurons both tested by sex pheromone and electrical stimulation.Electrical stimulation elicited similar response of neuron as blend,which was one kind of biphasic response pattern consisting of a depolarization phase and followed by a hyperpolarization phase.The depolari zation phase stimulated by electrical shock was very short,but it was clearly present in this and all other tested neurons.In all tested neurons stimulated by electrical shock,there were 2-10 spikes followed the stimulus artifact and 10-80 ms burst duration in the depolarization phase.So electrical stimulation elicit ed the frequency response of neuron as same as the pulsed response of neuron activated by blend.Hyperpolarization potential and followed inhibition duration bec ame bigger and longer with hyperpolarizing current injection.This was different from the case in Manduca sexta,which the early inhibitory potential was re versed with only -0.2 nA of injected current.Further analysis revealed that the frequency of neuron positively correlation with the hyperpolarization potential (R=0.667,P<0.0001) and negatively correlation with inhibition duration (R=-0.709,P<0.0001).Results indicated descriptor of neurons in MGC with electrical stimulation in A.segetum should be proved by more experiments.

     

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