Abstract: The mitochondrial DNAs (mtDNAs) from liver of C.auratus red var.,F1 hybrids of red crucian carp (♀)×common carp (♂),C.auratus cuvieri and C.auratus auratus and ovary of C.auratus auratus were isolated and purified by the means of differential centrifugation and nuclease digestion.After having been digested by 9 restriction endonucleases (EcoRⅠ,HindⅢ,PstⅠ,BglⅡ,BamHⅠ,XhoⅠ,XbaⅠ,SalⅠ and KpnⅠ),the mtDNAs were analysed by using agarose gel electrophoresis.The results showed that PstⅠ,KpnⅠ and BglⅡ were the restriction fragments length polymorphism among four strains,but without the polymorphism within strains.The mitochondrial DNA size was about 16.19 kb in C.auratus red var,16.02 kb in hybrids,16.60 kb in C.auratus cuvieri and 16.06 kb in C.auratus auratus respectively.According the proportion of shared restriction fragments,the genetic distances between strains were calculated.The results manifested that the genetic distance was the smallest between C.auratus red var.and its filial generation hybrids,and that mtDNA followed the character of matrilinear inheritance.