Abstract: Eighteen restriction endonucleases,ApaⅠ,BamHⅠ,BclⅠ,BglⅠ,BglⅡ,ClaⅠ,DraⅠ,EcoRⅠ,EcoRⅤ,HaeⅡ,HindⅢ,KpnⅠ,PstⅠ,PvuⅡ,SacⅠ,SalⅠ,SmaⅠ and XhoⅠwere used to investigate the mitochondrial DNA polymorphism of different goat breeds from Europe,Africa and China.Among the 33 individuals of 5 goat breeds analyzed in this paper,27 restriction morphs were detected which could be sorted into 8 haplotypes.The results indicated that the main haplotype of internal goat breeds was Ⅰ and Ⅲ,but Ⅳ for goat breeds of Europe and Africa.It was pointed out that the goat breeds analyzed might originate from two different maternal side.Meanwhile,the molecular dendrogram of different populations showed that the internal goat breeds had the close relationship,so did the European and African goat breeds.It was also verified that the Aferican goat breed,Nubian goat was once introduced into the European goat breed,Sanne goat.