鲹科鱼类线粒体DNA控制区结构及系统发育关系
Mitochondrial DNA Control Region Structure and MolecularPhylogenetic Relationship of Carangidae
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摘要: 采用PCR技术获得了9种鲹科鱼类的线粒体DNA控制区全序列，并结合从GenBank中下载的3种鲹科鱼类的相应序列采用Clustal W排序后，对控制区结构进行分析，识别了其终止序列区、中央保守区和保守序列区3个区域，指出了终止相关序列的主体是TACAT与其反向互补序列ATGTA以及一系列保守序列（CSB-F、CSB-E、CSB-D和CSB-1、CSB-2、CSB-3），并给出了它们的一般形式，同时在康氏似鲹控制区的5′和3′两端发现重复序列。以尖吻鲈作为外类群，应用邻接法构建的分子系统树表明：鲹科鱼类分为鲹亚科、鰤亚科、鲳鲹亚科和鰆鲹亚科4个亚科，各自形成单系群；鲹亚科与鰤亚科形成姐妹群，鲳鲹亚科再与他们聚在一起，鰆鲹亚科处于鲹科鱼类的基部，与前面3个亚科聚在一起。Abstract: Complete sequences of the mitochondrial DNA control region from nine species of Carangidae were amplified by Polymerase Chain Reaction (PCR), and were aligned by Clustal W with three other species of Carangidae from GenBank. According to the alignment, three domains, the termination associated sequence domain (TAS), the central conserved domain (CD) and the conserved sequence block domain (CSB), were identified in the mtDNA control region of Carangidae. A termination associated sequence (TACAT) and its reverse complementary sequence (ATGTA) were found in the TAS domain. Three conserved blocks (CSB-F, CSB-E,CSB-D) in the CD domain and three conserved sequence blocks (CSB-1, CSB-2, CSB-3) in the CSB domain were also identified. Repeat sequences were found in the 5′ and 3′ ends of the control region in Scomberoides commersonianus. With Lates calcarifer as the outgroup, the molecular phylogenetic relationship of Carangidae was analyzed using the neighbor-joining (NJ) method in PAUP 4.0b 10. The results showed that Carangidae could be divided into four subfamilies: Caranginae, Seriolinae, Trachinotinae and Chorineminae. Each of these subfamilies formed a monophyly. Caranginae and Seriolinae formed a sister group, and Trachinotini was sister to the clade of Caranginae and Seriolinae. Chorineminae, which located in the base of the tree and was sister to the other three subfamilies.
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