Percoll等密度梯度离心法分离利什曼原虫细胞核
Isolation and Purification of Nuclei of Leishmania gerbilli by Percoll Isopycnic Density Gradients Centrifugation
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摘要: 分离纯化锥虫类原生动物细胞核存在着许多困难,阻碍了人们对这类单细胞生物的细胞核在各方面作进一步研究。西文提出一个从破碎细胞开始的分离核的完整程序。采用起始密度为1.083的Perool工作液作等密度梯度离心,获得了纯度较高的利什曼原虫(Leishmania gerbilli)细胞核,L.gerbilli细胞核在Percoll中的浮力密度为1.048-1.051。Abstract: A complete procedure of nuclei isolation and purification from Leishmania gerbilli was established.With this procedure highly purified nuclei of L.gerbilli can be obtained.The isopycnic density of the nuclei is about 1.048-1.051 in percoll media.