MNNG转化赤麂细胞株(KIZ-8401)的生长特性和核型观察

Observations on New Cell line of Indian muntjac Transformed With Mnng

摘要: 本文用MNNG （G-Methy-N-nitro-N-nitrosoguanidine)（甲基硝基亚硝基胍）转化赤麂肺成纤维细胞株（KIZ-7901）。转化的赤鹿细胞株（KIZ-8401）具有新的核型，2n=8♂，细胞形态，细胞周期以及生物学特性都与未转化的细胞株（2n=7♂）不一样。转化细胞中还常见多倍体出现，细胞具有克隆生长能力。

Abstract: Lung fibroblast line of Indian muntjac (KIZ-7901) was transformed with MNNG (N-Methyl-N-nitro-N-nitrosoguanidine).The transformed Indian muntjac cells (KIZ-8401) possessed the new karyotype with chromosome number of 8 in male and polyploid.The morphology and cell cycle as well as mitotic index in MNNG-transformed muntjac cells were different from diploid cell line of Indian muntjac (KIZ-7901).Moreover,the MNNG-transformed muntjac cells possessed colony-forming capacity in vitro.