Abstract: The cytogenetic study on the karyotypes,Ag-NORs,C-banding and other features on five pelobatoid toad species in China had been made using the method of vapour fixation of mitosis metaphase of the marrow cells.The results showed that Megophrys jingdongensis have 2n=26(20M+4SM+2ST),5+8,SC and Ag-NORs localized on 6pper at which C-positive staining also.The centromeric C-bands dominated.M.palpebralispinosa 2n=26(20M+6SM),5+8,6qinter is distinct Ag-staining.Except for Nos.12,13,which has obvious terminal C-staining,all the chromosomes presented centromeric C-bands.Ophryphyne microstoma 2n=26(24M+2SM),6+7,SC and Ag-NORs are on 5pinter.Specially there are quite developed C-bands on all the centromeric regions.Oreolalax lichuanensis 2n=26(22M+4SM),6+7,SC and Ag-NORs located on 6qinter.Leptobrachium chapaensis 2n=26(22M+4SM),6+7,same location of Ag-NORs and C-bands with the former species it has,the varied chromosomes (26,27,28) occurred,but the exceed chromosomes are without C-staining.No heteromorphic chromosomes related to the sexuality were found in three species with both sexes.