Abstract: 6S (α-amino propioni acid)-fluorescein isothiocyanate-progesterone conjugate (6NHPF) was prepared by the method of chemical synthesis and it was used for detection of progesterone receptors (PR) in the chick oviduct.The results have shown that PR exist in the nuclei of luminal epithelium but neither in the smooth muscle and nor in the excretive and reproductive cavity,Furthmore,taking progesterone or intermediate compound of fluorescent progesterone conjugate as PR competitors,the fluorescent progesterone conjugate was effectively competed in the neighbouring sections.Therefore,this new,hydrophious,small moleculer fluorescent progesterone conjugate was proved specifically combined with progesterone receptors.