Abstract: The isozyme phenotypes of lactate dehydrogenase (LDH),malate dehydrogenase (MDH),glucose-6-phosphate dehydrogenase (G-6-PDH) and esterase (EST) between gynogenetic haploid and diploid of Rana limnochris were compared by polyacrylamide gel slab-electrophoresis.The results show that there are many differences in activities and bands between their corresponding isozymes.With these results,we are of the opinion that the abnormal expression of the isozyme genes of the haploid genome is due to lack of the interaction through another genome.