蝌蚪(Bufo bufo andrewsi)血红细胞微核和

贺维顺; 王蕊芳

蝌蚪(Bufo bufo andrewsi)血红细胞微核和

Detection of Water Pollution By Micronuclei and Other Nuclear Anomalies of Erythrocytes of Tadpoies (Buro bufo andrewsi)

摘要

摘要: 滇池是云南高原最大的淡水湖，是昆明地区工农业生产及人民生活用水的水源，又是昆明市的重要风景区。近年来，滇池水质污染，越来越引起人们的关注。昆明市的工业废水和生活污水经大观河等流入滇池，其中有毒有害物质多达30多种，包括镉、汞、砷、铅、铬、氟、有机磷、氰货物等。其中砷（Léonard et al.,1980；Katsuhiko,et al.,1981；贺维顺等，1988），铅（刘明韬等，1983；黄旭培等，1984），铬（Léonard，1980；Nakamuro et al.,Wild，1978；贺维顺等，1987），氟（贺维顺等，1983），有机磷（贺维顺等，1982）等都是业经证实或可疑的致突变物。这些物质对人体健康的远期效应，尤其是潜在的致癌效应如何？是人们普遍关心的问题。利用蝌蚪血有核红细胞微核和核异常，来检测大观河污水的致突变性，以预测流入滇池的污染物对人体健康的潜在致癌性，是本工作的主要目的。

Abstract: These studies present a approach to analysis of micronuclei (MN) and other nuclear anomalies (ONA) in erythrocytes of toad (Bufo bufo andrewsi) tadpoles,induced by exposure to polluted water of Daguan River.Tadpoles were exposed to polluted water of three different concentration (33.3% 50.0% and 100.0%) for 7 days.In the control,total frequency of nuclear anomalies (including micronuclei) was 4.1‰;Total frequencies of ONA for the above mentioned experiment groups were 8.1‰,10.8‰ and 15.8‰ respectively.The frequencies of MN and ONA in erythrocytes of tadpole,in various groups,showed distinct dose dependent increases.Our results shown that MN and ONA in erythrocytes of toad tadpole is useful index for detecting genotoxic compounds in the aquatic environment.

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