浙江产眼镜蛇毒胆碱酯酶的研究Ⅲ、酶的疏水层析行为

Studies on the Cholinesterase From the Venom of Naja Naja Atra of Zhejiang Province Ⅲ.Some Hydrophobic Chromatographic Behaviour

摘要: 本文报导了三种疏水层析吸附剂的制备，并比较了这几种吸附剂对浙江产眼镜蛇毒胆碱酯酶的吸附情况，它们都能吸附这种酶、烷基越长吸附得越牢。经此疏水层析后的酶，在聚丙烯酰胺凝胶电泳中依旧有6条同式酶带、提示这些同工酶的疏水性质相似。

Abstract: This paper reported the preparation of three hydrophobic chromatographic absorbents and the comparison of some hydrophobic chromatographic behaviour of the cholinesterase from the Venom of Naja Naja atra.The length of the "Spacer arm" affected the adsorption of the enzyme,the more extended the carbon chain of the "Spacer arm",the stronger the adsorption of the enzyme.The cholinesterase from hydrophobic chromatography gave six bands in disc gel electrophoresis too.That implied a similar of the hydrophobic region of the isoenzymes.