眼镜王蛇蛇毒的柱层析分离及神经毒组份的研究

Column Chromatography Fractionation of Ophiophagus Hannan Venom and Study of Neurotoxic Peaks

摘要: 用羧甲基葡聚糖C-25和氯化钠直线梯度洗脱方法，对我国广西产眼镜王蛇蛇毒进行了柱层析分离，获得了17个蛋白峰，测定了17个蛋白峰的毒性和生理活性。结果表明有7个毒性峰，其中6个峰具有阻断神经-肌肉接头传递的作用，另一个峰毒性很小，在相同剂量下也不表现上述作用。

Abstract: We fractionated Chinese Guangxi Ophiophagus hannah venom by CM-Sephadex C-25 column chromatography.Linear gradient elution was carried out with NaCl.Seventeen protein peaks were obtained.Their toxicity and physiological activities were estimated.Of the seven toxic peaks,six had the effect of blockading the transmission of neuromuscle juncture.The other one was much less toxic.It didnt show such effect with the same dose.