Abstract: Antimicrobial peptides are distributed in animals and plants,and acted as the first defensive line against microorganisms.Compared with mammals,the acquired immune system in amphibians is very fragile.The innate system is mainly composed of antimicrobial peptides in amphibian skins that act as very important defensive function against the invasiveness of microorganisms including bacteria,fungi,virus and protozoa.A mini-review about the structure,function and application of amphibian antimicrobial peptides is reported in this paper.