• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
冯志国, 袁维佳, 章骏, 许燕. 2003: 钙离子浓度对光暗适应罗氏沼虾感光细胞可溶性Gq蛋白α亚基的影响. 动物学研究, 24(5): 373-376.
引用本文: 冯志国, 袁维佳, 章骏, 许燕. 2003: 钙离子浓度对光暗适应罗氏沼虾感光细胞可溶性Gq蛋白α亚基的影响. 动物学研究, 24(5): 373-376.
FENG Zhi-guo, YUAN Wei-jia, ZHANG Jun, XU Yan. 2003. Effects of Calcium Concentration on the Soluble Gq Protein α Subunit in the Photoreceptor Cell of Macrobrachium rosenbergi on Light Adaptation and Dark Adaptation. Zoological Research, 24(5): 373-376.
Citation: FENG Zhi-guo, YUAN Wei-jia, ZHANG Jun, XU Yan. 2003. Effects of Calcium Concentration on the Soluble Gq Protein α Subunit in the Photoreceptor Cell of Macrobrachium rosenbergi on Light Adaptation and Dark Adaptation. Zoological Research, 24(5): 373-376.

钙离子浓度对光暗适应罗氏沼虾感光细胞可溶性Gq蛋白α亚基的影响

Effects of Calcium Concentration on the Soluble Gq Protein α Subunit in the Photoreceptor Cell of Macrobrachium rosenbergi on Light Adaptation and Dark Adaptation

  • 摘要: 以光适应和暗适应条件下的罗氏沼虾复眼视网膜为材料,分别用高钙溶液、生理溶液、低钙溶液孵育后,用蛋白质提取液提取可溶性Gq蛋白α亚基并SDS-PAGE电泳,利用Tanon GIS凝胶图像处理系统分析其含量。从光暗条件下3种溶液孵育后的感光细胞中都提取到了42 kDa的可溶性Gq蛋白α亚基。高钙溶液、生理溶液、低钙溶液孵育后的感光细胞中可溶性Gq蛋白α亚基的含量,光适应组分别是3.13%、3.09%、3.03%;暗适应组分别是3.17%、3.06%、3.01%。在生理溶液和低钙溶液中,该蛋白含量光适应组大于暗适应组,但低钙溶液的光适应组与暗适应组无显著差异;在高钙溶液中,暗适应组显著大于光适应组,可能与钙离子反馈调节作用有关。

     

    Abstract: After light and dark adaptation,the retina of the Macrobrachium rosenber was respective incubated in high calcium solution,physiological solution and low calcium solution.Soluble Gq α was extracted from the retina of the Macrobrachium rosenber with protein extract and was electophoresised by SDS-PAGE.The percent of the soluble Gq α was analyzed by Tanon GIS gel image disposal system.After the incubation in three solutions,the soluble Gq α in 42 kDa was all extracted from photoreceptor cell.The percent of the soluble Gq α in photoreceptor cell in high calcium solution,physiological solution and low calcium solution was 3.13%,3.09% and 3.03% respectively on light adaptation and was 3.17%,3.06% and 3.01% respectively on dark adaptation.In physiological solution and low calcium solution,the percent of the protein on light adaptation was higher than that on dark adaptation,but in low calcium solution,the percent of the protein on light and dark adaptation was no significantly different;in high calcium solution,the percent of the protein on dark adaptation was higher than that on light adaptation.This was probably relevant to feedback regulation.

     

/

返回文章
返回