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Novel astrovirus and paramyxovirus in Mongolian gerbils (Meriones unguiculatus) from China

Shou-Min Nie Juan Li Yi-Ting Wang Cui-Hong An Hong Zhou Lin Xu Yang-Xin Sun Wen-Hui Chang Ci-Xiu Li Wei-Feng Shi

Shou-Min Nie, Juan Li, Yi-Ting Wang, Cui-Hong An, Hong Zhou, Lin Xu, Yang-Xin Sun, Wen-Hui Chang, Ci-Xiu Li, Wei-Feng Shi. Novel astrovirus and paramyxovirus in Mongolian gerbils (Meriones unguiculatus) from China. Zoological Research, 2022, 43(3): 418-422. doi: 10.24272/j.issn.2095-8137.2021.445
Citation: Shou-Min Nie, Juan Li, Yi-Ting Wang, Cui-Hong An, Hong Zhou, Lin Xu, Yang-Xin Sun, Wen-Hui Chang, Ci-Xiu Li, Wei-Feng Shi. Novel astrovirus and paramyxovirus in Mongolian gerbils (Meriones unguiculatus) from China. Zoological Research, 2022, 43(3): 418-422. doi: 10.24272/j.issn.2095-8137.2021.445

长爪沙鼠中新型星状病毒和副粘病毒的鉴定

doi: 10.24272/j.issn.2095-8137.2021.445

Novel astrovirus and paramyxovirus in Mongolian gerbils (Meriones unguiculatus) from China

Funds: This study was supported by the Academic Promotion Programme of Shandong First Medical University (2019QL006), and the Open Research Fund Program of CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology (2021SPCAS002)
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  • 摘要: 长爪沙鼠(Meriones unguiculatus)是鼠科的小型啮齿类动物,广泛分布于中国北方的荒漠草原地区,但很少有在野生长爪沙鼠中发现病毒的报道。利用宏转录组测序技术,课题组从陕西省鼠疫疫区的长爪沙鼠中发现了两种新型RNA病毒,分别命名为Gerbil astrovirus (GeAstV)(沙鼠星状病毒)和Gerbil paramyxovirus (GePV)(沙鼠副粘病毒)。基于ORF1a、RdRp和ORF2氨基酸序列的系统发育分析表明,GeAstV与啮齿类和猪相关星状病毒关系最为密切,其中RdRp与猪星状病毒的氨基酸序列同源性最高,为75.07%,而ORF1a和ORF2蛋白与一种啮齿类星状病毒同源性分别为42.14%和35.94%,提示可能存在重组和潜在的跨物种传播事件。对于新发现的GePV,进化分析表明与Tailam virus和Beilong virus关系密切,其中L蛋白与Jeilongvirus属7种病毒的同源性为61%-77%。值得注意的是,GeAstV仅在肺标本中检测到,GePV仅在肾标本中检测到。总之,该研究证明了两种新的沙鼠RNA病毒的存在,加深了人们对野生长爪沙鼠携带病毒多样性的认识。
    #Authors contributed equally to this work
  • Figure  1.  Genome characterization and evolutionary relationships of GeAstV and GePV

    A: Schematic representation and read abundance of the genome of GeAstV. The characteristic amino acid motifs are highlighted with red arrows. B–D: Maximum likelihood phylogenies of GeAstV and representative members of the genus Mamastrovirus were inferred based on the amino acid alignments of ORF1a (B), RdRp (C), and ORF2 (D). The two gerbil astrovirus sequences are highlighted in red with red solid circles. The grey-shaded regions show the sequences with phylogenetic incongruence and the rodent associated astrovirus. E: Maximum likelihood phylogeny of the L protein of gerbil paramyxovirus and the representative members of the Paramyxoviridae, including those from the genera Ferlavirus, Aquaparamyxovirus, Respirovirus, Henipavirus, Narmovirus, Salemvirus, Morbillivirus, and Jeilongvirus. The rodent associated viruses are highlighted with a grey background. F: The genome of GePV and nine paramyxoviruses from the genus Jeilongvirus: Beilong virus (BeiPV), Tailam virus (TlmPV), Rodent paramyxovirus (RoPV), Pohorje myodes paramyxovirus 1 (PMPV-1), Jun jeilongvirus (JPV), Mount Mabu Lophuromys virus 1 (MMLV-1) and 2 (MMLV-2), Feline paramyxovirus(FePV), and Ruloma virus. Read abundance of the genome of GePV are shown on the top, followed by open reading frames (ORFs) colored according to their putative functions. The numbers to the right of each virus indicate the length of the virus genome. The genomes are drawn to a unified length scale shown at the bottom. Homologous gene regions are connected by dotted lines. B–E: The trees were midpoint rooted for clarity. Bootstrap values of >70% are shown for key nodes.

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  • ZR-2021-445 Supplementary Materials.pdf
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出版历程
  • 收稿日期:  2022-02-07
  • 录用日期:  2022-04-11
  • 网络出版日期:  2022-04-15
  • 刊出日期:  2022-05-18

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