高质量染色体水平的斑鰶(Konosirus punctatus)参考基因组为其适应性进化提供了见解
Chromosome-level genome assembly of the dotted gizzard shad (Konosirus punctatus) provides insights into its adaptive evolution
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摘要: 斑鰶是一种经济上重要的海洋渔业资源,广泛分布于从印度洋到太平洋的海域。它是盐度和温度适应性遗传研究的良好非模式物种。然而,高质量的参考基因组尚未被报道。该研究使用 Illumina、Pacific Biosciences 和 Hi-C 测序技术组装了一个 800.00 Mb 的高质量染色体水平的基因组,contig N50 长度为 2.14 Mb。组装的序列通过 Hi-C 数据锚定到 24 条假染色体上。共预测了 24298 个蛋白质编码基因,其中 91.08% 被成功注释为假定功能。这一新的高质量斑鰶参考基因组为更深入地了解温度和盐度适应以及物种保护提供了基础资源。Abstract: Konosirus punctatus is an economically important marine fishery resource and is widely distributed from the Indian to Pacific oceans. It is a good non-model species for genetic studies on salinity and temperature adaptation. However, a high-quality reference genome has not yet been reported. Here, an 800.00 Mb high-quality chromosome-level genome with a contig N50 length of 2.14 Mb was assembled using Illumina, Pacific Biosciences, and Hi-C sequencing technology. The assembled sequences were anchored to 24 pseudochromosomes by the Hi-C data. In total, 24 298 protein-coding genes were predicted, 91.08% of which were successfully annotated with putative functions. Furthermore, 587 putative genes were identified as being under positive selection. This new high-quality K. punctatus reference genome provides a fundamental resource for a deeper understanding of temperature and salinity adaptation and species conservation.