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何婉韵, 张星星, 高国龙, 高鸣艺, 钟发刚, 吕鲁超, 蔡钟鹏, 斯幸峰, 杨军, 刘健华. 2021: 携带fosA3blaCTX-M-14的O101:H9-ST10和O101:H9-ST167型大肠杆菌在中国养殖场腹泻犊牛中的克隆传播,其中O101:H9-ST10型大肠杆菌可能来源于澳大利亚红嘴鸥. 动物学研究, 42(4): 461-468. DOI: 10.24272/j.issn.2095-8137.2021.153
引用本文: 何婉韵, 张星星, 高国龙, 高鸣艺, 钟发刚, 吕鲁超, 蔡钟鹏, 斯幸峰, 杨军, 刘健华. 2021: 携带fosA3blaCTX-M-14的O101:H9-ST10和O101:H9-ST167型大肠杆菌在中国养殖场腹泻犊牛中的克隆传播,其中O101:H9-ST10型大肠杆菌可能来源于澳大利亚红嘴鸥. 动物学研究, 42(4): 461-468. DOI: 10.24272/j.issn.2095-8137.2021.153
Wan-Yun He, Xing-Xing Zhang, Guo-Long Gao, Ming-Yi Gao, Fa-Gang Zhong, Lu-Chao Lv, Zhong-Peng Cai, Xing-Feng Si, Jun Yang, Jian-Hua Liu. 2021: Clonal spread of Escherichia coli O101:H9-ST10 and O101:H9-ST167 strains carrying fosA3 and blaCTX-M-14 among diarrheal calves in a Chinese farm, with Australian Chroicocephalus as the possible origin of E. coli O101:H9-ST10. Zoological Research, 42(4): 461-468. DOI: 10.24272/j.issn.2095-8137.2021.153
Citation: Wan-Yun He, Xing-Xing Zhang, Guo-Long Gao, Ming-Yi Gao, Fa-Gang Zhong, Lu-Chao Lv, Zhong-Peng Cai, Xing-Feng Si, Jun Yang, Jian-Hua Liu. 2021: Clonal spread of Escherichia coli O101:H9-ST10 and O101:H9-ST167 strains carrying fosA3 and blaCTX-M-14 among diarrheal calves in a Chinese farm, with Australian Chroicocephalus as the possible origin of E. coli O101:H9-ST10. Zoological Research, 42(4): 461-468. DOI: 10.24272/j.issn.2095-8137.2021.153

携带fosA3blaCTX-M-14的O101:H9-ST10和O101:H9-ST167型大肠杆菌在中国养殖场腹泻犊牛中的克隆传播,其中O101:H9-ST10型大肠杆菌可能来源于澳大利亚红嘴鸥

Clonal spread of Escherichia coli O101:H9-ST10 and O101:H9-ST167 strains carrying fosA3 and blaCTX-M-14 among diarrheal calves in a Chinese farm, with Australian Chroicocephalus as the possible origin of E. coli O101:H9-ST10

  • 摘要: 在对2018年从中国新疆某养殖场分离的犊牛腹泻源大肠杆菌进行耐药性监测时,发现其对磷霉素耐药率异常高(48.5%),该研究旨在揭示其磷霉素耐药基因及其传播机制。聚合酶链式反应(PCR)结果显示,所有磷霉素耐药大肠杆菌均携带fosA3基因。脉冲场凝胶电泳(PFGE)和 Southern 印迹杂交结果显示,16 株fosA3阳性分离株属于四种不同的PFGE 谱型(即A、B、C、D)。11株D谱型克隆株的fosA3基因位于染色体上,而其余fosA3基因则由质粒携带。对全基因组测序和长读长测序结果进行分析发现,D谱型菌株为O101:H9-ST10型大肠杆菌,C、B、A谱型菌株分别为O101:H9-ST167、O8:H30-ST1431和未知ST的O101:H9型大肠杆菌。在 C谱型菌株中,fosA3基因与blaCTX-M-14基因共存于F18:A-:B1质粒上。有趣的是,基于核心基因组单核苷酸多态性(cgSNPs)的系统发育分析表明,O101:H9-ST10型大肠杆菌与同样携带fosA3基因和blaCTX-M-14基因的澳大利亚红嘴鸥源O101:H9-ST10型大肠杆菌密切相关,二者仅相差11个cgSNP。这些结果表明,O101:H9-ST10型高风险耐药大肠杆菌可能通过候鸟跨陆地传播。

     

    Abstract: During a 2018 antimicrobial resistance surveillance of Escherichia coli isolates from diarrheal calves in Xinjiang Province, China, an unexpectedly high prevalence (48.5%) of fosfomycin resistance was observed. This study aimed to reveal the determinants of fosfomycin resistance and the underlying transmission mechanism. Polymerase chain reaction (PCR) screening showed that all fosfomycin-resistant E. coli carried the fosA3 gene. Pulsed-field gel electrophoresis (PFGE) and southern blot hybridization revealed that the 16 fosA3-positive isolates belonged to four different PFGE patterns (i.e., A, B, C, D). The fosA3 genes of 11 clonally related strains (pattern D) were located on the chromosome, while others were carried by plasmids. Whole-genome and long-read sequencing indicated that the pattern D strains were E. coli O101:H9-ST10, and the pattern C, B, and A strains were O101:H9-ST167, O8:H30-ST1431, and O101:H9 with unknown ST, respectively. Among the pattern C strains, the blaCTX-M-14 gene was co-localized with the fosA3 gene on the F18:A-:B1 plasmids. Interestingly, phylogenetic analysis based on core genome single nucleotide polymorphisms (cgSNPs) showed that the O101:H9-ST10 strains were closely related to a Australian-isolated Chroicocephalus-origin E. coli O101:H9-ST10 strain producing CTX-M-14 and FosA3, with a difference of only 11 SNPs. These results indicate possible international dissemination of the high-risk E. coli clone O101:H9-ST10 by migratory birds.

     

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