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惠航博, 肖玲, 孙伟, 周英杰, 张海艳, 葛楚天. 2021: Sox9是温度依赖型性别决定的红耳龟睾丸分化的必需基因. 动物学研究, 42(6): 721-725. DOI: 10.24272/j.issn.2095-8137.2021.136
引用本文: 惠航博, 肖玲, 孙伟, 周英杰, 张海艳, 葛楚天. 2021: Sox9是温度依赖型性别决定的红耳龟睾丸分化的必需基因. 动物学研究, 42(6): 721-725. DOI: 10.24272/j.issn.2095-8137.2021.136
Hang-Bo Hui, Ling Xiao, Wei Sun, Ying-Jie Zhou, Hai-Yan Zhang, Chu-Tian Ge. 2021: Sox9 is indispensable for testis differentiation in the red-eared slider turtle, a reptile with temperature-dependent sex determination. Zoological Research, 42(6): 721-725. DOI: 10.24272/j.issn.2095-8137.2021.136
Citation: Hang-Bo Hui, Ling Xiao, Wei Sun, Ying-Jie Zhou, Hai-Yan Zhang, Chu-Tian Ge. 2021: Sox9 is indispensable for testis differentiation in the red-eared slider turtle, a reptile with temperature-dependent sex determination. Zoological Research, 42(6): 721-725. DOI: 10.24272/j.issn.2095-8137.2021.136

Sox9是温度依赖型性别决定的红耳龟睾丸分化的必需基因

Sox9 is indispensable for testis differentiation in the red-eared slider turtle, a reptile with temperature-dependent sex determination

  • 摘要: SOX9(SRY-related HMG box gene 9)是脊椎动物物种雄性性别决定和睾丸分化的重要调控因子。然而,Sox9在爬行动物睾丸分化的功能研究尚未见报导。该研究发现产雄温度下的红耳龟(Trachemys scripta)胚胎中Sox9缺失导致完全的性逆转,其性腺出现卵巢样的结构,雄性标记蛋白AMH消失,雌性标记蛋白FOXL2出现表达,生殖细胞也呈现雌性分布模式。相反,在产雄温度下的红耳龟胚胎中过表达Sox9导致不完全的性逆转,性腺中AMH和FOXL2共存。我们的研究首次直接证明了Sox9对于爬行动物物种的睾丸分化是必不可少的,进一步证实Sox9在脊椎动物雄性发育中的保守作用。

     

    Abstract: SOX9 (SRY-related HMG box gene 9) is an essential regulator of male sex determination and testis differentiation in many vertebrate species. However, the functional role of Sox9 in testis differentiation has not yet been identified in any reptilian species. Herein, Sox9 knockdown in the red-eared slider turtle (Trachemys scripta) embryos at a male-producing temperature led to complete male-to-female sex reversal, characterized by the formation of an ovary-like structure, disappearance of male marker AMH, and ectopic expression of ovarian regulator FOXL2, as well as a female distribution pattern of germ cells. Conversely, in-ovo overexpression of Sox9 at a female-producing temperature resulted in partial masculinization of putative female embryos, with the co-existence of AMH and FOXL2. Our study provides the first direct evidence that Sox9 is indispensable for testicular differentiation in a reptilian species, further confirming the conserved role of Sox9 in vertebrate sexual development.

     

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