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严超超, 张新尚, 周亮, 杨巧, 周敏, 张琳婉, 邢津川, 闫志峰, MeganPrice, 李静, 岳碧松, 范振鑫. 2020: 衰老对圈养猕猴血液转录组的影响及其与人类的比较. 动物学研究, 41(5): 557-563. DOI: 10.24272/j.issn.2095-8137.2020.092
引用本文: 严超超, 张新尚, 周亮, 杨巧, 周敏, 张琳婉, 邢津川, 闫志峰, MeganPrice, 李静, 岳碧松, 范振鑫. 2020: 衰老对圈养猕猴血液转录组的影响及其与人类的比较. 动物学研究, 41(5): 557-563. DOI: 10.24272/j.issn.2095-8137.2020.092
Chao-Chao Yan, Xin-Shang Zhang, Liang Zhou, Qiao Yang, Min Zhou, Lin-Wan Zhang, Jin-Chuan Xing, Zhi-Feng Yan, Megan Price, Jing Li, Bi-Song Yue, Zhen-Xin Fan. 2020: Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans. Zoological Research, 41(5): 557-563. DOI: 10.24272/j.issn.2095-8137.2020.092
Citation: Chao-Chao Yan, Xin-Shang Zhang, Liang Zhou, Qiao Yang, Min Zhou, Lin-Wan Zhang, Jin-Chuan Xing, Zhi-Feng Yan, Megan Price, Jing Li, Bi-Song Yue, Zhen-Xin Fan. 2020: Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans. Zoological Research, 41(5): 557-563. DOI: 10.24272/j.issn.2095-8137.2020.092

衰老对圈养猕猴血液转录组的影响及其与人类的比较

Effects of aging on gene expression in blood of captive Tibetan macaques (Macaca thibetana) and comparisons with expression in humans

  • 摘要: 衰老一直是亟待解决的科学难题。藏酋猴已经作为一些人类重大疾病的动物模型,比如青光眼、糖尿病和器官移植。本文重点研究了衰老对藏酋猴血液转录组产生的影响,并且将其结果与衰老对人类血液转录组所产生的影响进行比较,比较了藏酋猴在此方面与人类的异同。我们从不同年龄的24只藏酋猴个体鉴定出1,358个显著上调差异表达基因(DEGs)和1,165个显著下调的DEGs。通过WGCNA鉴定得到两个与年龄显著相关的模块,其中的核心基因(core gene)有大部分属于DEGs。进一步分析确定出藏酋猴应对衰老的潜在重要通路,FoxO signaling pathway和B cell receptor signaling pathway。在藏酋猴和人类的对比分析中,鉴定出279个藏酋猴与人类共享的年龄相关DEGs,其中27个在两个物种的表达不同。例如在B cell receptor signaling pathway中起着激活B细胞下游体液免疫的INPPL1基因,在人类中随年龄增长表达量上调,但在藏酋猴中随年龄增长表达量下调。本文对年龄在藏酋猴血液组织基因表达中的作用进行了研究,发现了藏酋猴与人类在重要调控通路中表达模式异同的基因,为藏酋猴未来更好地充当疾病模式动物提供了参考。

     

    Abstract: Changes in gene expression occur as animals, including primates, age. Macaques have long been used as a model species for primate evolution and biomedical studies. Here, to study gene expression in Tibetan macaques (Macaca thibetana, TMs) and its differences to humans, we applied RNA-Seq to obtain the blood transcriptomes of 24 TMs. In total, 2 523 age-associated differentially expressed genes (DEGs) were identified. Several pathways and processes that regulate aging, including the FoxO signaling pathway, autophagy, and platelet activation, were significantly enriched in the up-regulated DEGs. Two significantly age-related modules were identified by weighted gene co-expression network analysis (WGCNA). The TMs and humans shared 279 common DEGs, including 111 up-regulated and 141 down-regulated genes with advancing age in the same expression direction. However, 27 age-related DEGs presented the opposite expression direction in TMs as that in humans. For example, INPPL1, with inhibitory effects on the B cell receptor signaling pathway, was up-regulated in humans but down-regulated in TMs. In general, our study suggests that aging is a critical factor affecting gene expression in the captive TM population. The similarities and differences in gene expression patterns between TMs and humans could provide new insights into primate evolution and benefit TM model development.

     

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