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唐艺, 辛鸽, 赵玲敏, 黄力行, 覃映雪, 苏永全, 郑炜强, 吴斌, 林楠, 鄢庆枇. 2020: 应用基于时间序列的双物种转录组学研究大黄鱼与变形假单胞菌之间的宿主-病原互作. 动物学研究, 41(3): 314-327. DOI: 10.24272/j.issn.2095-8137.2020.035
引用本文: 唐艺, 辛鸽, 赵玲敏, 黄力行, 覃映雪, 苏永全, 郑炜强, 吴斌, 林楠, 鄢庆枇. 2020: 应用基于时间序列的双物种转录组学研究大黄鱼与变形假单胞菌之间的宿主-病原互作. 动物学研究, 41(3): 314-327. DOI: 10.24272/j.issn.2095-8137.2020.035
Yi Tang, Ge Xin, Ling-Min Zhao, Li-Xing Huang, Ying-Xue Qin, Yong-Quan Su, Wei-Qiang Zheng, Bin Wu, Nan Lin, Qing-Pi Yan. 2020: Novel insights into host-pathogen interactions of large yellow croakers (Larimichthys crocea) and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens. Zoological Research, 41(3): 314-327. DOI: 10.24272/j.issn.2095-8137.2020.035
Citation: Yi Tang, Ge Xin, Ling-Min Zhao, Li-Xing Huang, Ying-Xue Qin, Yong-Quan Su, Wei-Qiang Zheng, Bin Wu, Nan Lin, Qing-Pi Yan. 2020: Novel insights into host-pathogen interactions of large yellow croakers (Larimichthys crocea) and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens. Zoological Research, 41(3): 314-327. DOI: 10.24272/j.issn.2095-8137.2020.035

应用基于时间序列的双物种转录组学研究大黄鱼与变形假单胞菌之间的宿主-病原互作

Novel insights into host-pathogen interactions of large yellow croakers (Larimichthys crocea) and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens

  • 摘要: 宿主与病原之间的相互作用非常复杂,在感染过程中涉及基因表达的大量动态变化。这些相互作用对于理解宿主的抗感染能力以及病原体的致病机理是至关重要的。由于病原在感染组织中的载菌量很低,限制了双物种转录组测序在细菌性病原与动物宿主的互作研究的应用。本研究建立了变形假单胞菌(Pseudomonas plecoglossicida)-大黄鱼(Larimichthys crocea)的急性感染模型,病原在脾脏中有较高的载菌量。受感染鱼的脾脏表现出典型症状,并且在感染后2天(dpi)时细菌载量达到最大。对受感染大黄鱼的脾脏进行病原与宿主双物种转录组测序,并与PBS注射的大黄鱼脾脏和体外培养的细菌的转录组数据进行比较。结果表明,病原体与宿主之间的相互作用是高度动态调控的,在感染过程中,大黄鱼和变形假单胞菌转录组会发生相对应的变化。在感染期间,大黄鱼涉及细胞因子-细胞因子受体,Toll样受体信号传导和其他免疫相关途径的许多基因的表达水平显着上调。此外,变形假单胞菌感染严重影响了大黄鱼的代谢过程和氧气的使用。对于变形假单胞菌,运动相关基因和鞭毛组装相关基因的表达水平显着上调。应用WGCNA进行时序分,析结果表明,代谢过程与整个免疫过程密切相关。这项研究的结果有助于阐明在感染期间大黄鱼和变形假单胞菌之间的相互作用。

     

    Abstract: Host-pathogen interactions are highly complex, involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, time-resolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection (dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study host-pathogen interactions between L. crocea and P. plecoglossicida. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor, Toll-like receptor signaling, and other immune-related pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune process. For P. plecoglossicida, the expression levels of motility-related genes and flagellum assembly-related genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and P. plecoglossicida.

     

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