良好的肠道完整性及低微生物易位有助于SIVmac239感染的北平顶猴维持低

Ming-Xu Zhang; Tian-Zhang Song; Hong-Yi Zheng; Xue-Hui Wang; Ying Lu; Han-Dan Zhang; Ting Li; Wei Pang; Yong-Tang Zheng

doi:10.24272/j.issn.2095-8137.2019.047

良好的肠道完整性及低微生物易位有助于SIVmac239感染的北平顶猴维持低

doi: 10.24272/j.issn.2095-8137.2019.047

张明旭^1,2,
宋天章^1,2,
郑宏毅¹,
王雪卉^1,3,
路莹^1,2,
张瀚丹¹,
李婷¹,
庞伟¹,
郑永唐^1,4,

1.
中国科学院昆明动物研究所中国科学院动物模型与人类疾病机理重点实验室/云南省生物活性肽重点实验室，昆明非人灵长类高等级生物安全研究中心，中国科学院昆明动物研究所-香港中文大学生物资源与疾病分子机理联合实验室，云南昆明 650223，中国
2.
中国科学院大学昆明生命科学学院，云南昆明 650204，中国
3.
中国科学技术大学生命科学学院，安徽合肥 230026，中国
4.
苏州大学药学院中国科学院昆明动物研究所-苏州大学动物模型与药物开发联合实验室，江苏苏州 215123，中国

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出版历程
- 收稿日期: 2018-12-13
- 录用日期: 2019-03-07
- 刊出日期: 2019-10-14

Superior intestinal integrity and limited microbial translocation are associated with lower immune activation in SIVmac239-infected northern pig-tailed macaques (Macaca leonina)

1.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, National Kunming High Level Biosafety Research Center for Non-human Primates, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
2.
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming Yunnan 650204, China
3.
School of Life Sciences, University of Science and Technology of China, Hefei Anhui 230026, China
4.
KIZ-SU Joint Laboratory of Animal Models and Drug Development, College of Pharmaceutical Sciences, Soochow University, Suzhou Jiangsu 215123, China

Funds: This work was partly supported by grants from the National Natural Science Foundation of China(U1802284);13th Five-Year Key Scientific and Technological Program of China(2017ZX10304402-002-004);Knowledge Innovation Program of the Chinese Academy of Sciences(ZDRW-ZS-2016-4)

More Information

Corresponding author: Yong-Tang Zheng

摘要

摘要: 微生物易位导致HIV及SIV感染后系统性免疫活化是艾滋病发病的重要原因之一。我们发现在SIVmac239感染期间，北平顶猴CD8⁺ T细胞活化水平显著低于中国恒河猴。另外，其血浆脂多糖结合蛋白及可溶性CD14水平同样低于中国恒河猴。相较于中国恒河猴，北平顶猴的Chiu评分明显更低，这意味着其能维持更好的肠道完整性。无论在SIV感染或未感染的北平顶猴回肠和结肠上皮层均未发现明显的损伤，这一点不同于中国恒河猴。而且，在SIV感染或未感染的北平顶猴回肠和结肠部位均未检测到明显的微生物易位。以上研究结果表明，北平顶猴在SIV感染期间维持良好的肠道完整性及低水平的微生物易位可能是其CD8⁺ T细胞活化水平更低的重要原因。
- 免疫活化 /
- 肠道完整性 /
- 微生物易位 /
- SIVmac239 /
- 北平顶猴(Macaca leonine) /
- 中国猕猴(Macaca mulatta)
Abstract: Microbial translocation is a cause of systemic immune activation in HIV/SIV infection. In the present study, we found a lower CD8⁺ T cell activation level in Macaca leonina (northern pig-tailed macaques, NPMs) than in Macaca mulatta (Chinese rhesus macaques, ChRMs) during SIVmac239 infection. Furthermore, the levels of plasma LPS-binding protein and soluble CD14 in NPMs were lower than those in ChRMs. Compared with ChRMs, SIV-infected NPMs had lower Chiu scores, representing relatively normal intestinal mucosa. In addition, no obvious damage to the ileum or colon epithelial barrier was observed in either infected or uninfected NPMs, which differed to that found in ChRMs. Furthermore, no significant microbial translocation (Escherichia coli) was detected in the colon or ileum of infected or uninfected NPMs, which again differed to that observed in ChRMs. In conclusion, NPMs retained superior intestinal integrity and limited microbial translocation during SIV infection, which may contribute to their lower immune activation compared with ChRMs.
- Immune activation /
- Intestinal integrity /
- Microbial translocation /
- SIVmac239 /
- Macaca leonina /
- Northern pig-tailed macaques