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Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles

De-Li Xu Meng-Meng Xu De-Hua Wang

De-Li Xu, Meng-Meng Xu, De-Hua Wang. Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles. Zoological Research, 2019, 40(4): 305-316. doi: 10.24272/j.issn.2095-8137.2019.045
Citation: De-Li Xu, Meng-Meng Xu, De-Hua Wang. Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles. Zoological Research, 2019, 40(4): 305-316. doi: 10.24272/j.issn.2095-8137.2019.045


doi: 10.24272/j.issn.2095-8137.2019.045

Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles

Funds: This study was supported by the National Natural Science Foundation of China (31770444) and the State Key Laboratory of Integrated Management of Pest Insects and Rodents (IPM1906)
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    Corresponding author: De-Hua Wang
  • 摘要: 环境温度是影响动物许多生理功能如抗氧化能力和免疫的重要因子,抗氧化防御和免疫能力在一定程度上可以反映动物的生存能力。我们以布氏田鼠(Lasiopodomys brandtii)为研究对象,检验高温或低温会抑制其抗氧化防御和天然免疫能力的假说。将30只雄性布氏田鼠随机分为4°C (n=10)、23°C (n=10)和32 °C 组(n=10),处理27天。结果发现低温显著增加了总能量摄入(GEI)以及肝脏、心脏和肾脏鲜重,但降低了总体脂重和胴体干重。随着温度的降低,反映活性氧(ROS)水平的过氧化氢(H2O2)浓度在肝脏中升高,心脏中降低,而在肾脏、睾丸和小肠中没有变化。不同温度组肝脏、心脏、肾脏、睾丸和小肠中反映脂质过氧化程度的丙二醛(MDA)含量均无显著性差异,暗示着高温和低温并没有造成氧化损伤。除了低温增加睾丸过氧化氢酶(CAT)活性外,所检测的上述5种组织中超氧化物歧化酶(SOD)、CAT和总抗氧化能力(T-AOC)均不受低温或高温的影响。与23 °C组相比,4°C组反映天然免疫的血清杀菌能力几乎受到抑制,而脾脏和白细胞总数不受温度处理的影响。温度处理影响睾酮水平,但不影响皮质酮浓度,并且这两种激素与5种组织中的天然免疫、H2O2和MDA浓度、SOD、CAT和 T-AOC活性均不存在相关性。总之,这些结果表明温度对布氏田鼠的氧化应激、抗氧化酶活性和免疫作用具有组织特异性。抗氧化防御能力的上调或维持可能是布氏田鼠在温度高度可变的环境中生存的重要机制。
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  • 收稿日期:  2018-12-30
  • 刊出日期:  2019-07-18