• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
向诚, 李志娜, 黄田庄, 李经辉, 杨雷, 魏景宽. 2019: 小鼠三个早期发育阶段最大电惊厥阈值(MEST)的异同. 动物学研究, 40(3): 231-235. DOI: 10.24272/j.issn.2095-8137.2019.038
引用本文: 向诚, 李志娜, 黄田庄, 李经辉, 杨雷, 魏景宽. 2019: 小鼠三个早期发育阶段最大电惊厥阈值(MEST)的异同. 动物学研究, 40(3): 231-235. DOI: 10.24272/j.issn.2095-8137.2019.038
Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. 2019: Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice. Zoological Research, 40(3): 231-235. DOI: 10.24272/j.issn.2095-8137.2019.038
Citation: Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. 2019: Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice. Zoological Research, 40(3): 231-235. DOI: 10.24272/j.issn.2095-8137.2019.038

小鼠三个早期发育阶段最大电惊厥阈值(MEST)的异同

Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

  • 摘要: 人类和其他哺乳动物的大脑,在出生后仍会经历快速的生长发育,并在此过程中逐渐形成完善的大脑功能。本研究利用最大电惊厥阈值检测范式,对产后早期(1-2周龄)、离乳期(3周龄)和青春早期(4-5周龄)的未成年小鼠,开展诱发癫痫大发作行为实验,探索不同发育阶段大脑的神经电生理特点。我们将0.5-4.0 mA、35-200 V范围内不同参数搭配的电刺激给予不同周龄的小鼠后,观察造成其最大电惊厥(MES)、无响应和致死等三种行为学表现的电流、电压范围。结果显示:相较于青春早期小鼠,产后早期小鼠在更宽的电流电压范围(1.0-4.0 mA,60-200V)内均可诱发MES,且无死亡;而离乳期小鼠仅在1.5-2.0 mA,100-160V内发生MES反应,致死范围较其他两组大。此外,产后早期MES反应表现为四肢弯曲,角弓反张,抽搐,与其他两组小鼠躯干和后肢强直,僵硬的行为不一致。总之,本研究通过诱导最大电惊厥阈值实验,发现出生后早期阶段神经系统对电刺激具有更高敏感性和更强耐受性;离乳期时存在关键神经生理调整期。这些结果提示,对于一些儿童多发神经系统疾病的病理和药理机制研究,如儿童癫痫等,应注意区分动物的发育时间,建立更加适用的幼年动物疾病模型。

     

    Abstract: Early brain development after birth is extremely dynamic, suggesting that potential functional changes occur during this period. In this study, the maximal electroshock seizure threshold (MEST) was used to explore the electrophysiological variation among three developmental stages in young mice (no more than 5 weeks old). The induced electroshock seizure (ES) behavior of early postnatal mice (1–2-weeks old) differed from that during weaning (3 weeks old) and early puberty (4–5-weeks old). Thus, we further explored their respective characteristic responses to the ES parameters. When the stimulation current (SC) was limited to 4.0 mA, only the 1–2-week-old mice were induced to exhibit ES behavior at voltages of 30 V and 40 V, indicating they were more sensitive to maximal electroshock seizure (MES) (response to lower voltage). Surprisingly, however, they showed substantially lower mortality than the older groups under higher voltage conditions (60, 100, 160, and 200 V), suggesting better tolerance to the SC. We also found that when the current limit decreased to 3.5 mA, the 4–5-week-olds mice exhibited stable ES behavior with low mortality, while for 3-week-olds mice, the SC limit required to be reduced to 1.5 mA. In conclusion, our findings showed that neural sensitivity to MES was significantly different in young mice before puberty. Thus, greater attention should be given to distinguishing the developmental period of mice, especially in electrophysiological examination.

     

/

返回文章
返回