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Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway

Jian-Peng Chen Wei Pang Zi-Wei Zhao Yan-Hui Bi Xiao-Wu Chen

Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028
Citation: Jian-Peng Chen, Wei Pang, Zi-Wei Zhao, Yan-Hui Bi, Xiao-Wu Chen. Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway. Zoological Research, 2019, 40(4): 337-342. doi: 10.24272/j.issn.2095-8137.2019.028

败血症金鱼的皮肤和头肾转录组及TLR通路分析

doi: 10.24272/j.issn.2095-8137.2019.028

Transcription profiles of skin and head kidney from goldfish suffering hemorrhagic septicemia with an emphasis on the TLR signaling pathway

Funds: This study was supported by a grant from the Key Project of Scientific and Technological Innovation of Blue Granary (2018YFD0901505)
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    Corresponding author: Xiao-Wu Chen
  • 摘要: 金鱼是常见的观赏鱼,也是中国较早就开始人工养殖的鱼类。影响金鱼外观的疾病能引起人们更多的重视。出血性败血症是一种影响金鱼的皮肤外观且高度致命的疾病。为了获得更多关于出血性败血症相关的免疫基因信息,我们对患有出血性败血症金鱼的皮肤(SK)和头肾(HK)进行了转录组测序,组装和比较,分别获得了来自SK和HK的28 646 938bp和34 682 151bp的原始数据。在此基础上,进一步组装成30 119条unigenes。功能注释后,在SK和HK中均获得各自不同的基因表达谱。SK和HK比较显示分别有122个和77个 unigenes的上调或下调2倍以上且P值小于0.05。很多免疫基因在SK和HK中均有表达,这些基因来自不同的免疫通路。比如Toll-like受体信号通路就有57个基因在SK和HK中被检测到。该通路相关的10个干扰素调剂因子和Toll-like受体都比较保守,在多种组织中表达。其中干扰素调节因子3(IRF3)是一个关键的干扰素转录调控因子。金鱼患败血症以及活体注射Poly I:C后都能引起IRF3上调。体外细胞学实验证明IRF3能通过增加含有IFN-β和ISRE顺式作用元件的干扰素启动子活性来调节干扰素表达。因此,金鱼IRF3是一个干扰素调节因子,在金鱼败血症中发挥抗病的功能。本研究结果将有助于对败血症鱼类免疫反应中起重要作用的基因进行筛选和分析。
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出版历程
  • 收稿日期:  2018-12-11
  • 刊出日期:  2019-07-18

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