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Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis

Fen Zhang Zhi-Lai Guo Yan Chen Li Li Hai-Ning Yu Yi-Peng Wang

Fen Zhang, Zhi-Lai Guo, Yan Chen, Li Li, Hai-Ning Yu, Yi-Peng Wang. Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis. Zoological Research, 2019, 40(3): 198-204. doi: 10.24272/j.issn.2095-8137.2018.070
Citation: Fen Zhang, Zhi-Lai Guo, Yan Chen, Li Li, Hai-Ning Yu, Yi-Peng Wang. Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis. Zoological Research, 2019, 40(3): 198-204. doi: 10.24272/j.issn.2095-8137.2018.070

C-末端酰胺化和七肽环对新型崑嵛林蛙(Rana kunyuensis)抗菌肽amurin-9KY生物学活性和高级结构的影响

doi: 10.24272/j.issn.2095-8137.2018.070
详细信息
    通讯作者:

    于海宁, 王义鹏

Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis

Funds: This work was supported by grants from the National Natural Science Foundation of China (31772455), Natural Science Foundation of Jiangsu Province (BK20160336 and BK20171214), Natural Science Foundation of College in Jiangsu Province (16KJB350004), Suzhou Science and Technology Development Project (SYN201504 and SNG2017045)
More Information
    Corresponding author: Hai-Ning Yu, Yi-Peng Wang
  • 摘要: 崑嵛林蛙(Rana kunyuensis)作为林蛙属的一种,仅自然生活于中国山东省烟台市昆嵛山境内。在本研究中,从合成的崑嵛林蛙皮肤cDNA文库中克隆获得一条279 bp的cDNA序列,该序列编码一种新型抗菌肽分子,命名为amurin-9KY。Amurin-9KY前体由62个氨基酸残基组成,其中成熟肽由14个氨基酸残基组成,包括由2个半胱氨酸残基形成的分子内七肽环和酰胺化的C-末端。这些结构特点表明amurin-9KY是一种新型两栖类抗菌肽家族成员之一。尽管amurin-9KY和之前从黑龙江林蛙(Rana amurensis)中发现的amurin-9AM具有较高的相似性,但是到目前为止对于amurin-9家族抗菌肽的结构和功能仍然未知。在本论文中,我们设计合成了amurin-9KY和3个改造体多肽amurin-9KY1-3,研究了C-末端酰胺化和七肽环对amurin-9KY结构和功能的影响。结果表明C-末端酰胺化对amurin-9KY的抗菌活性十分重要,而C-末端酰胺化和七肽环对amurin-9KY的溶血活性同时具有影响。圆二色谱(Circular dichroism, CD)结果表明上述4种多肽在TFE/H2O (v/v 1:1)溶液中均为α-螺旋型结构,而在水溶液中为无规则结构。打断七肽环的二硫键使2个半胱氨酸的巯基游离能够显著增加amurin-9KY的抗氧化活性。扫描电子显微镜(Scanning electron microscopy, SEM)同时也被用于研究amurin-9KY的抗菌机理。
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出版历程
  • 收稿日期:  2018-06-20
  • 刊出日期:  2019-05-18

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