• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
何大健, 王林, 张志毕, 郭琨, 李竞争, 和协超, 崔清华, 郑萍. 2018: 小鼠卵母细胞中母源基因 Ooep 可能参与同源重组介导的DNA双链损伤修复过程. 动物学研究, 39(6): 387-395. DOI: 10.24272/j.issn.2095-8137.2018.067
引用本文: 何大健, 王林, 张志毕, 郭琨, 李竞争, 和协超, 崔清华, 郑萍. 2018: 小鼠卵母细胞中母源基因 Ooep 可能参与同源重组介导的DNA双链损伤修复过程. 动物学研究, 39(6): 387-395. DOI: 10.24272/j.issn.2095-8137.2018.067
Da-Jian He, Lin Wang, Zhi-Bi Zhang, Kun Guo, Jing-Zheng Li, Xie-Chao He, Qing-Hua Cui, Ping Zheng. 2018: Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes. Zoological Research, 39(6): 387-395. DOI: 10.24272/j.issn.2095-8137.2018.067
Citation: Da-Jian He, Lin Wang, Zhi-Bi Zhang, Kun Guo, Jing-Zheng Li, Xie-Chao He, Qing-Hua Cui, Ping Zheng. 2018: Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes. Zoological Research, 39(6): 387-395. DOI: 10.24272/j.issn.2095-8137.2018.067

小鼠卵母细胞中母源基因 Ooep 可能参与同源重组介导的DNA双链损伤修复过程

Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes

  • 摘要: 卵母细胞中DNA损伤的发生可能导致不育和生殖缺陷。而DNA损伤中双链损伤(DSB)是最具伤害性的,它的存在甚至能伤及基因组的完整性。同源重组修复方式(HR)介导的DNA双链损伤修复过程在保护卵的质量和数量方面起着重要作用。然而,有关卵中参与同源重组过程的重要蛋白还知之甚少。本文中,我们发现小鼠卵中特异基因Ooep,可能是同源重组过程的关键组分。在应答DNA双链损伤发生的过程中,OOEP蛋白能有效地促进ATM蛋白的激活,使其与RAD51蛋白迅速积累于损伤部位。Ooep基因的缺失会破坏小鼠卵中DNA双链损伤修复能力,当外部损伤因素存在时会导致卵母细胞凋亡。此外,Ooep基因缺失的卵母细胞体外成熟过程会延迟。由此看来,OOEP蛋白是通过维持基因组稳定性的方式来保护卵母细胞质量和数量的。随着小鼠年龄的增加,Ooep基因表达水平也逐步下降,说明它还可能参与母源衰老过程。

     

    Abstract: DNA damage in oocytes can cause infertility and birth defects. DNA double-strand breaks (DSBs) are highly deleterious and can substantially impair genome integrity. Homologous recombination (HR)-mediated DNA DSB repair plays dominant roles in safeguarding oocyte quantity and quality. However, little is known regarding the key players of the HR repair pathway in oocytes. Here, we identified oocyte-specific gene Ooep as a novel key component of the HR repair pathway in mouse oocytes. OOEP was required for efficient ataxia telangiectasia mutated (ATM) kinase activation and Rad51 recombinase(RAD51)focal accumulation at DNA DSBs. Ooep null oocytes were defective in DNA DSB repair and prone to apoptosis upon exogenous DNA damage insults. Moreover, Ooep null oocytes exhibited delayed meiotic maturation. Therefore, OOEP played roles in preserving oocyte quantity and quality by maintaining genome stability. Ooep expression decreased with the advance of maternal age, suggesting its involvement in maternal aging.

     

/

返回文章
返回