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淡水育珠蚌池蝶蚌亲环蛋白D的特征性描述

Characterization of cyclophilin D in freshwater pearl mussel (Hyriopsis schlegelii)

  • 摘要: 亲环蛋白D(HsCypD)从淡水育珠蚌池蝶蚌中获得,其cDNA全长为2671bp,编码含367个氨基酸的蛋白。HsCypD被证实是一种含有10个磷酸化位点和四个肽重复序列结构域(TPR)的亲水性胞内蛋白。该蛋白含有一个核心序列区域YKGCIFHRIIKDFMVQGG,在脊椎动物和无脊椎动物中这个核心序列高度保守。系统进化树分析表明来自不同物种的CypD具有共同的祖先。此外,HsCypD与霸王莲花青螺CypD亲缘关系最近。HsCypD基因mRNA的表达水平呈现一定的组织多样性,肠内表达水平最高,其次是性腺,血细胞中表达最低。

     

    Abstract: Cyclophilin D (referred to as HsCypD) was obtained from the freshwater pearl mussel (Hyriopsis schlegelii). The full-length cDNA was 2 671 bp, encoding a protein consisting of 367 amino acids. HsCypD was determined to be a hydrophilic intracellular protein with 10 phosphorylation sites and four tetratricopeptide repeat (TPR) domains, but no signal peptide. The core sequence region YKGCIFHRIIKDFMVQGG is highly conserved in vertebrates and invertebrates. Phylogenetic tree analysis indicated that CypD from all species had a common origin, and HsCypD had the closest phylogenetic relationship with CypD from Lottia gigantea. The constitutive mRNA expression levels of HsCypD exhibited tissue-specific patterns, with the highest level detected in the intestines, followed by the gonads, and the lowest expression found in the hemocytes.

     

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