Abstract: The study dealt with the biological characteristics and behavior of adult serpentine leafminer Liriomyza huidobrensis (Blanchard) in Kunming,Yunnan Province.Activities,emergence,feeding,oviposition and longevity of adults were studied.Behavior of probing,mating,feeding and oviposition were described.Before feeding,females use tarsus,ovipositor and proboscis to assess the suitability of host plants.When the population density is high,some males attack other coupled males.During courtship,males scrape stridulatory apparatus to attract mating.The results of this study will provide some valuable information for future control methods such as plantresistance,genetic control and mating disturbance.