We record Theloderma pyaukkya for the first time in China based on one specimen collected from western Yunnan. Morphologically, the individual from Yunnan agrees well with the original description of T. pyaukkya and phylogenetically, it clustered together with type specimens including holotype of T. pyaukkya from Kachin State (northern Myanmar) with strong support. The taxonomic status of T. pyaukkya from Chin State (western Myanmar) needs further examination. In addition, we record Theloderma moloch in Yunnan for the first time. Thus, there have been seven Theloderma species recorded in Yunnan, China, namely Theloderma albopunctatum, T. baibungense, T. bicolor, T. gordoni, T. moloch, T. pyaukkya, and T. rhododiscus.