德宏水牛微卫星标记分析的群体遗传变异
Unfolding of Population Structure in Dehong Buffalo Using Microsatellite DNA Markers
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摘要: 德宏水牛是云南省地方水牛的优良品种之一，为了进一步阐明其群体遗传变异和遗传结构，筛选了分别位于水牛14条染色体上的15对微卫星引物，对德宏水牛81个个体进行了检测分析。共检测到62个等位基因，每个座位等位基因数目从2到6个不等，平均等位基因数为4.13，该水牛群体期望杂合度和多态信息含量分别为0.6520±0.1526和0.5863±0.1789，各座位的遗传分化系数在0～0.0919之间，平均值为0.0202。每个座位的基因流较大，平均12.1502。研究结果表明德宏水牛群体遗传多样性较丰富，亚群间的遗传分化程度低，基因流较大，且很少发生近交。Abstract: Dehong buffalo are an indigenous buffalo breed in Yunnan province, China. A total of 81 unrelated samples from Dehong buffalo breeds were genotyped using 15 microsatellite DNA markers from 14 chromosomes to estimate genetic diversity, genetic differentiation and gene flow within populations. A total of 62 alleles were detected across the 15 loci assayed. All the loci were polymorphic and the number of alleles ranged from two to six, giving a mean number of 4.13 alleles per locus. The expected heterozygosity and polymorphism information content of 15 microsatellite loci were 0.6520±0.1526 and 0.5863±0.1789, respectively. Coefficients of genetic differentiation ranged from 0 to 0.0919, with the average of 0.0202. Gene flow at each locus was large, with a mean Nm of 12.1502. The results indicated that the genetic diversity was rich among Dehong buffalo and the genetic differentiation in its sub-populations was relatively low, with larger gene flow and less inbreeding.
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