穴居狼蛛毒中磷酸单醌酶和核酸酶的研究
Study on phosphomonoesterase,Rnase and dnase in spider (Lycosa singoriensis) Venom
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摘要: 本文分别用对硝基酚磷酸酯、3-32p标记的RNA和大肠杆菌噬菌体入DNA作底物测定了新疆产穴居狼蛛毒中与核酸代谢有关的酶,发现此毒中含有磷酸单酯酶、核糖核酸酶和脱氧核糖核酸酶。Abstract: Activities of phosphomonoesterase and nucleases hydrolysing RNA and DNA were presented in a solution of the crude venom from a spider (Lycosa singoriensis) collected in Xinjiang Region,China.The substrates for assaying these enzymes were 4-nitrophenyl phosphate disodium salt,3 terminally 32plabellod RNA and nonlabelled DNA respectively.After incubation of RNA and DNA with the venom solution,the products were detected by polyacrylamide gel or agarose gel electrophoresis.