一种新的制备哺乳动物精母细胞联会复合体的微铺展技术
A new micro-spreading Technique for Demonstrating synaptonemal Complexes in Mammalian spermatocytes
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摘要: 本文报道一种新的制备哺乳动物精母细胞联会复合体(SC)的微铺展法,与常规铺展法比较,具有简便易行,适用于野外工作等特点。此外,微铺展法不仅可用新鲜材料,而且还可用长期冷冻保存的材料来制备SC标本。Abstract: A new micro-spreading technique for demonstrating synaptonemal complexes (SC) has been described.This method gives more consistent results than the routine spreading technique.Furthermore,the ease and speed should allow succssful application of this new simple method to field cytogenetic work.In addition,our results also indicated that both fresh and long-time frozen spermatocytes in liquid nitrogen can be used for preparing SC.