原癌基因FOS蛋白、雌二醇及其受体在中国林蛙不同时期精巢中的表达
Expression of c-fos, Oestradiol and Oestrogen Receptor in the Testis of Rana chensinensis at Different Stages
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摘要: 用免疫细胞化学方法检测了原癌基因FOS蛋白、17β-雌二醇（E2）和雌激素受体（ER）在中国林蛙生精周期中不同时期精巢内的表达定位。结果显示：在中国林蛙生精周期的Ⅰ—Ⅴ期，E2和ER在精原细胞、精母细胞、精子细胞、精子、支持细胞和间质细胞内均有表达。在不同时期的精巢中，E2和ER在生精细胞的定位具有一致性：在Ⅱ—Ⅲ期，精子细胞的E2和ER阳性表达最强；在Ⅲ期，精子中E2和ER阳性反应强度显著高于Ⅳ—Ⅴ期（P＜0.01)。在生精周期的Ⅰ—Ⅴ期，支持细胞中，E2和ER表达强度经历由强减弱再增强的变化过程。在生精周期的Ⅲ期，间质细胞中E2和ER阳性反应强度高于其他各期。除精子外的生精细胞，支持细胞和间质细胞内均有FOS阳性反应，其表达强度呈现阶段性变化。Abstract: Localisation of FOS, 17β-oestradiol (E2) and oestrogen receptors (ER) was investigated in testes at different stages of the spermatogenetic cycle of Rana chensinensis, using immunocytochemistry. The results showed that E2 and ER are expressed in spermatogonia, spermatocytes, spermatids, spermatozoa, Sertoli cells and Leydig cells from stage Ⅰ to stage Ⅴ of spermatogenesis. A strong positive co-expression of E2 and ER was observed in spermatids in stage Ⅱ and stage Ⅲ. The staining intensity of E2 and ER in stage Ⅲ spermatozoa was significantly higher than in stages Ⅳ and Ⅴ (P＜0.01). Expression of E2 and ER in Sertoli cells appears in stage Ⅰ and decreases in intensity from stage Ⅱ to stage Ⅳ, but increases in stage Ⅴ. Expression of E2 and ER in Leydig cells was higher in stage Ⅲ than in other stages. FOS was localised in spermatogonia, spermatocytes, spermatids, Sertoli cells and Leydig cells but not spermatozoa. Expression of FOS showed stagespecific characteristics.
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