鲮鱼的微卫星位点筛选和群体遗传多样性初步分析(英文)
Isolation of Microsatellite DNA and Preliminary Genomic Analysis of Mud Carp (Cirrhina molitorella) (in English)
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摘要: 利用鲤科鱼类微卫星引物在鲮鱼中进行扩增,结果在24对引物中，13对引物能成功扩增，且在鲮鱼中的扩增产物表现稳定，其中11对有较高多态性,等位基因数在2—7个之间，扩增的条带符合孟德尔遗传规律。随后利用筛选的微卫星座位对鲮鱼野生和养殖群体遗传多样性进行了初步分析。分析结果显示：鲮鱼野生群体的平均等位基因数5.2个；观测杂合度在0.25与0.8之间，平均观测杂合度(Ho)是0.61±0.2，平均期望杂合度(He)是0.8±0.09；群体座位平均多态信息含量(PIC)为0.72±0.1。相比之下，养殖群体的平均观测杂合度(Ho)和平均期望杂合度(He)都低于野生群体，分别是0.59±0.2、0.75±0.1。两群体间的遗传相似度为0.7774、遗传距离为0.2518。研究表明：用其他鱼类分离出的微卫星引物可以快速筛选到适用于鲮鱼遗传分析的微卫星座位。Abstract: In order to determine the applicability of microsatellite primers developed from common carp (Cyprinuscario) for genomic analysis in mud carp (Cirrhina olitorella), 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers (54%) successfully amplified specific products in the mud carp and 11 primers (48%) showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity (Ho), unbiased expected heterozygosity (He) and polymorphism information content (PIC) in the wild population were 0.61±0.2, 0.8±0.09 and 0.72±0.1 respectively. Several Hardy-Weinberg departure value were significantly departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input.
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