利用COI基因序列分析长江与澜沧江水系日本沼虾群体的遗传结构(英文)
Genetic Structure of the Oriental River Prawn (Macrobrachium nipponense) from the Yangtze and Lancang Rivers Inferred from COI Gene Sequence (in English)
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摘要: 测定了我国长江水系和澜沧江水系的日本沼虾9个群体，共79个个体的线粒体COI基因序列片段（约450 bp），结果发现有89个变异位点，共计有46个单倍型。其中云南昆明（KM）群体具有较丰富的遗传多样性（h=1.000，π=0.028），而重庆（CQ）群体的遗传多样性最小（h=0.700，π=0.008）。AMOVA分析表明，群体间的遗传变异占总遗传变异的9.66%，而90.34%的遗传变异源于群体内。采用邻接法（NJ）构建的分子系统树显示，46个单倍型明显地聚为长江中下游和长江上游与澜沧江两个族群。其结果可以为合理开发和利用日本沼虾自然野生资源，以及建立和保护日本沼虾种质资源库及基因库提供必要的参考。Abstract: This study analyzed nucleotide sequences from the mitochondrial cytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn (Macrobrachium nipponense) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations,the population from Kunming shows the greatest level of variability (h=1.000,π=0.028), whereas the population from Chongqing exhibits the lowest level of variability (h=0.700,π=0.008). Analysis of molecular variance suggested that of the total genetic diversity,9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (NJ) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.
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