Abstract: The fragments of the mitochondrial ND1 and COI gene of 20 Chinese species in the subfamily Elymninae were newly amplified and sequenced in this study. Combining the homologous sequences of 4 foreign species from GenBank and using 3 species of the families Papilionidae and Parnassiidae as the outgroups, the phylogenetic trees of these Elymninae groups were reconstructed with neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesina inference (BI) methods. The results showed that the tribes Zetherini and Elymniini are closely related to each other. The tribe Lethini is not monophyletic, with the genera Lethe and Neope are closely related to the tribe Mycalesini, the genera Chonala, Tatinga, Lasiommata and Pararge form a monophyletic clade, and what is more, the Chonala and Tatinga clustered together in all the trees with support of 100% bootstrap value or 1.00 posterior probability, and should be consequently grouped into one genus.