浙江蝮蛇蛇毒蛋白水解酶(蛋白酶a)的酶学性质及生物学活性研究

Enzymatic Properties and Biological Activities of A Proteolytic Enzyme (Proteinase A) From The Venom of Agkistrodon Halys Pallas

摘要: 应用离子交换及凝胶柱层析，从浙江蝮蛇蛇毒中分离纯化出一种具有酪蛋白水解活性的蛋白酶a。经研究表明，以酪蛋白为底物，该蛋白酶a作用的最适pH值为9.5，最适温度为45 ℃，Km值为1.33×10-5 mol/L，酶活性受EDTA抑制。蛋白酶a不水解TAME，BAEE等精氨酸脂。实验表明，蛋白酶a具有纤维蛋白溶解作用而无出血毒性，EDTA和半胱氨酸可抑制其纤溶活性。

Abstract: A proteolytic enzyme from the venom of Agkistrodon halys Pallas has been purified by ion-exchang chromatography and by gel-filtration.This proteolytic enzyme (called proteinase a) can hydrolyze casein.Using casein as the substrate,the optimum pH is about 9.5,the optimum temperature is about 45℃,and Km=1.33×10-5 mol/L.Its activity toward casein was inhibited by EDTA.It did not act on TAME and BAEE.Proteinase a showed fibrinolytic activity in vitro,EDTA and cysteine could inhibite this activity.