Abstract: A new freshwater goby, Rhinogobius wuyiensis sp. nov., was found in the Wuyi river（28°55′N，119°50′E）in Wuyi, Zhejiang province, China. The spot pattern on head of this species was similar to R. lentiginis, but differed from this species by a combination of characters: head with cephalic lateralis canals; vertebral count 10+16=26; anal fin has 7～9 branched fin-rays; first dorsal fin in adult male with elongate filamentous rays; head with irregular spots which usually join together；branchiostegal memberane rare with dots in male.