四种圆斑蝰蛇毒对血小板聚集抑制作用的比较研究

Comparative Studies of The Inhibition of Four Vipera russelli Venoms on Platelet Aggregation

摘要: 对四种不同来源的RVV对ADP、Col和Ris诱导的血小板聚集抑制作用进行比较研究。RVV-B、RVVS和RVV-Styp对三种诱导剂的血小析聚集均有抑制作用，而RVV-T虽有ADP和Col抑制作用，但缺乏对Ris的抑制活性。预先与RVV孵育的血小板经洗涤后重新悬浮于自身PPP中，与RVV-Styp孵育的血小板完全恢复对Ris的凝集反应，而与RVV-B和RVV-S孵育的血小板仅部分恢复其反应。上述结果表明，在RVV中可能含有二种不同的血小板聚集抑制活性，而其抑制机理可能牵涉到血小板、血浆等因素。

Abstract: The inhibition of RVV from different source on platelet aggregation induced by ADP,Collagen or Ristocetin were studied.RVV-B,RVV-S and RVV-Styp could inhibited aggregation induced by above reagents.RVV-T could inhibited aggregation induced by ADP and Collagen,but not by Ristocetin.Platelets preincubated with RVV were resuspended in PPP after washed.It was observed that platelets preincubated with RVV-Styp could entirely restored to respond to Ristocetin,but only partially with RVV-B and RVV-S.These results shown that two distinct platelet inhibitory activities in RVV exist,the mechanism of the inhibition could involved platelet,plasma and other factors.