牛蛙(Rana catesbeiana)染色体高分辨带显示的初步探讨
Preliminary Study of High-Resolution Banding of the Rana catesbeiana
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摘要: 本文报道显示牛蛙染色体高分辨带的一种方法。通过2—巯基乙醇的预处理和Ohnuki低渗液和低渗及水焰干燥可使染色体伸长并显出高分辨带纹,且这种带纹和人的高分辨G带类似。初步探讨了牛蛙染色体高分辨显带机理。Abstract: In this paper,A method,pre-trcatment with 2-Mercapto Ethanol and hypotonic treatment with the Ohnukis solution and flame dry,is employed to show chromosome high-resolution bands of Rana catesbeiana.This procedure can elongate chromosomes and leads the chromosomes to show highresolution bands which are similar to the high-resolution G-bands of human.The mechanism of chromosome high-resolution banding of Rana catesbeiana was discussed.