小鼠杂交细胞生物学特征的观察

The Observation of Biological Characteristics of Hybrid Cell in Mice

摘要: 本文用小鼠NS-1骨髓瘤细胞与小鼠脾淋巴细胞经PEG介导进行融合，通过HAT选择，有成效地获得了同种杂种细胞。在融合后第30、50、70、90天观察了杂种细胞的染色体畸变和间期细胞核损伤，并对亲本和杂种细胞的染色体核型、带型（G带、C带）进行了比较。

Abstract: Cell fusion between mouse myeloma NS-1 and mouse spleen cells was induced by polyethylene glycol (PEG).Hyrid cells were successfully obtained by HAT medium.The hybridcell population were examined 30,50,70 and 90 days after fusion,The G-,C- banding and the frequencies of chromosomal aberrations in parental and hybrid cells were then compared.