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Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti

ZHOU Rui-Xue MENG Tao Meng Hai-Bo HENG Dun-Xue BIN Shi-Yu CHENG Jia FU Gui-Hong CHU Wu-Ying * ZHANG Jian-She *

ZHOU Rui-Xue , MENG Tao, Meng Hai-Bo, HENG Dun-Xue , BIN Shi-Yu , CHENG Jia, FU Gui-Hong, CHU Wu-Ying, *, ZHANG Jian-She , *. Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti. Zoological Research, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141
Citation: ZHOU Rui-Xue , MENG Tao, Meng Hai-Bo, HENG Dun-Xue , BIN Shi-Yu , CHENG Jia, FU Gui-Hong, CHU Wu-Ying, *, ZHANG Jian-She , *. Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti. Zoological Research, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141

鳜鱼基因表达转录分析中的内参选择比较(英文)

doi: 10.3724/SP.J.1141.2010.02141
详细信息
    通讯作者:

    褚武英,张建社

Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti

More Information
    Corresponding author: CHU Wu-Ying, ZHANG Jian-She
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出版历程
  • 收稿日期:  2009-07-21
  • 修回日期:  1900-01-01
  • 刊出日期:  2010-04-22

Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti

doi: 10.3724/SP.J.1141.2010.02141
    通讯作者: CHU Wu-Ying, ZHANG Jian-She

摘要: 目前基因表达的转录分析多采用单一或多个看家基因作为内参来校正目的基因的表达量。该实验以鳜鱼6个不同组织和5个不同胚胎发育阶段为研究对象,应用实时荧光定量PCR技术,观察了GAPDH、β-actin和18S rRNA三个看家基因mRNA水平的表达情况。geNorm统计分析表明,胚胎发育阶段β-actin表达最为稳定;不同的组织样品间,GAPDH表达最为稳定;而18S rRNA 的表达在不同的发育阶段不稳定。当利用多基因作为内参时,使用两个最稳定表达的看家基因即可对目的基因的表达进行准确校正。该结果证实了基因表达转录分析中内参基因选择的必要性,同时为鳜鱼等鱼类基因表达分析时内参基因的选择提供有价值的参考

English Abstract

周瑞雪, 蒙 涛, 孟海波, 陈敦学, 宾石玉, 成 嘉, 符贵红, 褚武英, 张建社, . 鳜鱼基因表达转录分析中的内参选择比较(英文)[J]. 动物学研究, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141
引用本文: 周瑞雪, 蒙 涛, 孟海波, 陈敦学, 宾石玉, 成 嘉, 符贵红, 褚武英, 张建社, . 鳜鱼基因表达转录分析中的内参选择比较(英文)[J]. 动物学研究, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141
ZHOU Rui-Xue , MENG Tao, Meng Hai-Bo, HENG Dun-Xue , BIN Shi-Yu , CHENG Jia, FU Gui-Hong, CHU Wu-Ying, *, ZHANG Jian-She , *. Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti. Zoological Research, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141
Citation: ZHOU Rui-Xue , MENG Tao, Meng Hai-Bo, HENG Dun-Xue , BIN Shi-Yu , CHENG Jia, FU Gui-Hong, CHU Wu-Ying, *, ZHANG Jian-She , *. Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti. Zoological Research, 2010, 31(2): 141-146. doi: 10.3724/SP.J.1141.2010.02141

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