Volume 24 Issue 2
Mar.  2003
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LI Zhao-min, ZHAO Xiao-fan, WANG Jin-xing, WANG Jia-bao, WANG Sheng-li. Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera. Zoological Research, 2003, 24(2): 140-143.
Citation: LI Zhao-min, ZHAO Xiao-fan, WANG Jin-xing, WANG Jia-bao, WANG Sheng-li. Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera. Zoological Research, 2003, 24(2): 140-143.

Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2003-04-22
  • After 6th-instar larva Helicoverpa armigera was fed on non-steroidal ecdysone agonist RH-2485,proteins and esterases in some tissues of them were examined,and the slices of body wall were observed with optical microscopes.SDS-PAGE resulted in that the expression of a major protein decreased in fat body and body wall of the group fed for 24 h and 48 h;Native-PAGE indicated that the expression of new esterases appeared in hemolymph and midgut of the group fed for 48 h.The body wall structure of the larva fed for 24 h showed some features of ecdysis (mesocuticle and endocuticle became vacuoles,and epicuticle and exocuticle separated from epidermis),and abnormal ecdysis (epidermis became obviously thinner than normal one).These results enhanced our knowledge of the mechanism of RH-2485 killing Helicoverpa armigera.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera

Abstract: After 6th-instar larva Helicoverpa armigera was fed on non-steroidal ecdysone agonist RH-2485,proteins and esterases in some tissues of them were examined,and the slices of body wall were observed with optical microscopes.SDS-PAGE resulted in that the expression of a major protein decreased in fat body and body wall of the group fed for 24 h and 48 h;Native-PAGE indicated that the expression of new esterases appeared in hemolymph and midgut of the group fed for 48 h.The body wall structure of the larva fed for 24 h showed some features of ecdysis (mesocuticle and endocuticle became vacuoles,and epicuticle and exocuticle separated from epidermis),and abnormal ecdysis (epidermis became obviously thinner than normal one).These results enhanced our knowledge of the mechanism of RH-2485 killing Helicoverpa armigera.

LI Zhao-min, ZHAO Xiao-fan, WANG Jin-xing, WANG Jia-bao, WANG Sheng-li. Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera. Zoological Research, 2003, 24(2): 140-143.
Citation: LI Zhao-min, ZHAO Xiao-fan, WANG Jin-xing, WANG Jia-bao, WANG Sheng-li. Effect of Non-steroidal Ecdysone Agonist (RH-2485) on the Expression of Proteins in Some Tissues and the Structure of Body Wall in 6th-instar Larva Helicoverpa armigera. Zoological Research, 2003, 24(2): 140-143.

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