Volume 24 Issue 2
Mar.  2003
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WANG Xian-chun, LIANG Song-ping. Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA. Zoological Research, 2003, 24(2): 121-126.
Citation: WANG Xian-chun, LIANG Song-ping. Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA. Zoological Research, 2003, 24(2): 121-126.

Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2003-04-22
  • In order to investigate the application of mass spectrometry (MS) in the renaturation and purification of synthetic peptides,ω-conotoxin MⅦA was chemically synthesized by a solid-phase method.The sample was oxidatively treated in a glutathione-containing buffer,and then purified by ion-exchange chromatography and RP-HPLC.The renaturation and purification efficiencies of synthesized ω-conotoxin MⅦA were subsequently analyzed by MALDI-TOF-MS and tandem electrospray mass spectrometry (ESI-MS/MS).Meanwhile,using an electrophysiological method,its biological activity was also measured.Mass spectrometric and electrophysiological analysis revealed that renatured and purified ω-conotoxin MⅦA possesses the same conformation and physiological activity as those of natural ω-conotoxin MⅦA.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA

Abstract: In order to investigate the application of mass spectrometry (MS) in the renaturation and purification of synthetic peptides,ω-conotoxin MⅦA was chemically synthesized by a solid-phase method.The sample was oxidatively treated in a glutathione-containing buffer,and then purified by ion-exchange chromatography and RP-HPLC.The renaturation and purification efficiencies of synthesized ω-conotoxin MⅦA were subsequently analyzed by MALDI-TOF-MS and tandem electrospray mass spectrometry (ESI-MS/MS).Meanwhile,using an electrophysiological method,its biological activity was also measured.Mass spectrometric and electrophysiological analysis revealed that renatured and purified ω-conotoxin MⅦA possesses the same conformation and physiological activity as those of natural ω-conotoxin MⅦA.

WANG Xian-chun, LIANG Song-ping. Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA. Zoological Research, 2003, 24(2): 121-126.
Citation: WANG Xian-chun, LIANG Song-ping. Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin MⅦA. Zoological Research, 2003, 24(2): 121-126.

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