Volume 24 Issue 2
Mar.  2003
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WEN Duan-cheng, BI Chun-ming, ZHU Zi-yu, LEI Lei, YANG Cai-xia, CHEN Da-yuan. Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos. Zoological Research, 2003, 24(2): 81-85.
Citation: WEN Duan-cheng, BI Chun-ming, ZHU Zi-yu, LEI Lei, YANG Cai-xia, CHEN Da-yuan. Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos. Zoological Research, 2003, 24(2): 81-85.

Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2003-04-22
  • To form tetraploid embryos,electric pulses were used to stimulate 2-cell blastomeres of Kunming mice.The pulses included combinations of four strengths (600,800,1000 and 1200 V/cm) and three durations (10,30 and 50 μs).Of the combinations,600 V/cm×50 μs and 1200 V/cm×30 μs produced the fusion rates of 91.6% and 93.0% respectively,and both were higher than the others.On the other hand,the pulses of 600 V/cm×10 μs and 1200 V/cm×50 μs did the rates of 58.9% and 60.2% respectively,being significantly lower than the others.The results showed that a certain pulse strength was necessary to achieve embryo fusion,and the duration was a key factor affecting the embryo fusion and its later development.What observed with a laser confocal microscope implied that the allocation,approach and fusion of nucleus was a spontaneous process in fused embryos.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos

Abstract: To form tetraploid embryos,electric pulses were used to stimulate 2-cell blastomeres of Kunming mice.The pulses included combinations of four strengths (600,800,1000 and 1200 V/cm) and three durations (10,30 and 50 μs).Of the combinations,600 V/cm×50 μs and 1200 V/cm×30 μs produced the fusion rates of 91.6% and 93.0% respectively,and both were higher than the others.On the other hand,the pulses of 600 V/cm×10 μs and 1200 V/cm×50 μs did the rates of 58.9% and 60.2% respectively,being significantly lower than the others.The results showed that a certain pulse strength was necessary to achieve embryo fusion,and the duration was a key factor affecting the embryo fusion and its later development.What observed with a laser confocal microscope implied that the allocation,approach and fusion of nucleus was a spontaneous process in fused embryos.

WEN Duan-cheng, BI Chun-ming, ZHU Zi-yu, LEI Lei, YANG Cai-xia, CHEN Da-yuan. Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos. Zoological Research, 2003, 24(2): 81-85.
Citation: WEN Duan-cheng, BI Chun-ming, ZHU Zi-yu, LEI Lei, YANG Cai-xia, CHEN Da-yuan. Forming Tetraploid Embryos with Electric Fusing Blastomeres of Kunming Mouse Embryos. Zoological Research, 2003, 24(2): 81-85.

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