Volume 23 Issue 4
Jul.  2002
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PAN Jian-yi, HU Wei-jun, LIANG Song-ping. Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana. Zoological Research, 2002, 23(4): 280-283.
Citation: PAN Jian-yi, HU Wei-jun, LIANG Song-ping. Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana. Zoological Research, 2002, 23(4): 280-283.

Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2002-08-22
  • A neurotoxin named Hainantoxin-Ⅵ (HNTX-Ⅵ) was purified from the venom of the hinese bird spider Selenocosmia hainana by ion exchange chromatography and r everse phase high performance liquid chromatography.The molecular weight of HNTX-Ⅵ is 3.99 849 kDa identified by MALDI-TOF mass spectrometry,which is in good agreement with the complete amino acid sequence of NH[2]-ECKYLWGTCEKDEH CCEHLGCNKKHGWCGWDGTF-COOHdetermined by automatic Edman degradation.The neurotoxin can paralyze rat after intracerebroventricular injection and block the neu romuscular transmission of the isolated rat phrenic nerve-diaphragm preparation .The 34-residue peptide,which contained 6 Cys and formed three disulfide bond s,showed high homology with two kinds of K[+] channel inhibitor,the HaTx from the venom of the Grammostola spatulata and the SGTx1 from the tarantula Scodra griseipes.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana

Abstract: A neurotoxin named Hainantoxin-Ⅵ (HNTX-Ⅵ) was purified from the venom of the hinese bird spider Selenocosmia hainana by ion exchange chromatography and r everse phase high performance liquid chromatography.The molecular weight of HNTX-Ⅵ is 3.99 849 kDa identified by MALDI-TOF mass spectrometry,which is in good agreement with the complete amino acid sequence of NH[2]-ECKYLWGTCEKDEH CCEHLGCNKKHGWCGWDGTF-COOHdetermined by automatic Edman degradation.The neurotoxin can paralyze rat after intracerebroventricular injection and block the neu romuscular transmission of the isolated rat phrenic nerve-diaphragm preparation .The 34-residue peptide,which contained 6 Cys and formed three disulfide bond s,showed high homology with two kinds of K[+] channel inhibitor,the HaTx from the venom of the Grammostola spatulata and the SGTx1 from the tarantula Scodra griseipes.

PAN Jian-yi, HU Wei-jun, LIANG Song-ping. Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana. Zoological Research, 2002, 23(4): 280-283.
Citation: PAN Jian-yi, HU Wei-jun, LIANG Song-ping. Purification,Sequencing and Characterization of Hainantoxin-Ⅵ,a Neuro toxin from the Chinese Bird Spider Selenocosmia hainana. Zoological Research, 2002, 23(4): 280-283.

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